Dernières publications

07Mai 2020

Skeletal muscles fibers are formed by the fusion of hundreds of cells where myonuclei are actively spread at myofibers periphery. Growing evidences supports connections between myonuclear positioning and muscle functionality. How myonuclei localization in muscle fibers contributes to muscle efficiency remains poorly characterized. Here, we show that in developing myofibers, SH3KBP1 scaffolds Endoplasmic Reticulum through […]

22Avr 2020

Skeletal muscle fibers are large syncytia but it is currently unknown whether gene expression is coordinately regulated in their numerous nuclei. By snRNA-seq and snATAC-seq, we showed that slow, fast, myotendinous and neuromuscular junction myonuclei each have different transcriptional programs, associated with distinct chromatin states and combinations of transcription factors. In adult mice, identified myofiber […]

22Avr 2020

Duchenne muscular dystrophy (DMD) is a devastating neuromuscular disease caused by an absence of the dystrophin protein, which is essential for muscle fiber integrity. Among the developed therapeutic strategies for DMD, the exon-skipping approach corrects the frameshift and partially restores dystrophin expression. It could be achieved through the use of antisense sequences, such as peptide-conjugated […]

25Fév 2020

Exercise training improves skeletal muscle function, notably through tissue regeneration by muscle stem cells. Here, we hypothesized that exercise training reprograms the epigenome of muscle cell, which could account for better muscle function. Genome-wide DNA methylation of myotube cultures established from middle-aged obese men before and after endurance exercise training identified a differentially methylated region […]

28Jan 2020

Macrophages are key immune cells in mediating both the acute inflammatory phase and the repair phase after tissue damage. Macrophages switch from pro-inflammatory to anti-inflammatory cells that sustain tissue repair and return to homeostasis. We show that the metabolic sensor, AMP-activated protein kinase (AMPK) is essential for glucocorticoid induction of an anti-inflammatory macrophage phenotype. While […]