Annonces professionnelles

Funded PhD project at IGBMC – Strasbourg – France

Project :
The PhD student will use next generation sequencing strategies, exome and genome sequencing of patients DNA, and RNAseq/transcriptome from patient muscles, and process these big datasets through bioinformatics pipelines to identify mutated genes in patients with myopathies. The functional impact of mutations will be confirmed by in vitro assays with recombinant proteins and transfected and differentiated muscle cells followed by confocal and video microscopies. The importance of these new myopathy genes for muscle function will be evaluated in vivo in an animal model (zebrafish or mice depending on the gene conservation) with CRISPR/Cas9 genome editing, RNA interference and adeno-associated virus. Animals will be phenotyped with simple motor tests, histology, electron microscopy and confocal imaging. This will also provide faithful laboratory models in which therapies may be later tested.
The success of this project will lead to a better diagnosis and patient care, and the identified genes will increase our understanding of the pathomechanisms and represent novel therapeutic targets.

Application:
Please send your CV, letters of recommendation and Master results to J Laporte (jocelyn@igbmc.fr) and J Bohm (johann@igbmc.fr)

Fore more details, click here.


Offre de thèse sur l’influence du sexe sur les mécanismes cellulaires impliqués dans la réparation musculaire post-lésionnelle (Université Claude Bernard Lyon I)

Un contrat doctoral est disponible à partir du 1er octobre 2020 pour caractériser l’influence du sexe sur les régulations cellulaires impliquées dans la réparation du tissu musculaire. Ce projet s’appuiera sur des protocoles standardisés de dommages musculaires induits par l’exercice et s’attachera à évaluer si et comment les atteintes fonctionnelles et la cinétique de régénération musculaire sont dépendantes du sexe. En parallèle, nous identifierons les mécanismes cellulaires et moléculaires sous-jacents en analysant les interactions cellulaires mises en jeu dans la régénération musculaire grâce à des approches in vitro (e.g., cultures cellulaires).
Les candidats doivent avoir un diplôme de Master en Sciences Biologiques, avec un bagage solide en biologie cellulaire et moléculaire.

Les candidat(e)s intéressé(e)s doivent soumettre leur candidature à Julien Gondin (julien.gondin@univ-lyon1.fr) avec leur CV, les informations d’au moins deux superviseurs de stage ou de formation, et une présentation concise de leur intérêt pour la recherche et leur objectifs professionnels.

Pour plus d’informations, cliquez ici.


PhD position: ‘Blood cells as a therapeutic tool to repair ailing muscles from heritable muscle
diseases’

Our laboratory is interested in understanding various aspects of early myogenic differentiation in the embryo using the avian (mainly) and rodent models. Our recent work has focused on the process of fusion, whereby myofibres become multinucleated. As a “spin-off” of those fundamental studies, we have initiated a translational program aimed at determining whether blood cells that have been genetically engineered to express the fusion master gene Myomaker (MYMK) can be used to repair dystrophic muscles. We obtained (unpublished) remarkable data showing that blood cells expressing MYMK, fuse to muscles in vitro and in vivo (both in WT and Mdx mice, a model of Duchenne Dystrophy). The research project aims at following a number of leads to significantly improve the efficiency of fusion to myofibres in adult mice. In the Mdx model, therapeutic blood cells will carry in addition to MYMK a gene repair toolkit (e.g. CRISPR/Cas9) aimed at correcting mutant nuclei. Molecular (dystrophin expression) and functional recovery will be monitored. This translational project combines innovative approaches and technologies to pave the way for the design of novel strategies to repair muscles of patients affected by hereditary diseases.

Please send your complete application documents (consisting of a CV, list of publications, letter of motivation, names of two references) to (christophe.marcelle@univ-lyon1.fr) by 31.07.2020.

For more details click here.


Postdoctoral Researcher

Closing Date: Thu 23 Jul 2020

Project: Dissecting AMPK/AMPK-related kinase signalling through Affinity-directed PROtein Missile (AdPROM) system

Applications are invited for a Postdoctoral Researcher to work in the laboratory of Gopal Sapkota at the Medical Research Council Protein Phosphorylation and Ubiquitylation Unit (MRC-PPU) in Dundee in close collaboration with the group of Kei Sakamoto at the Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, to uncover specific roles for AMP-activated protein kinase (AMPK) and its related kinases (with specific emphasis on salt inducible kinase (SIK) (Ref: PMID: 30385008)) signalling pathway in metabolic functions. Although much effort has been made to elucidate regulatory mechanism of the AMPK/AMPK-related kinases, their isoform-specific/cell-specific functions through regulating their distinct downstream targets are still elusive. This work will use state-of-the-art protein engineering technologies pioneered by the Sapkota lab to dissect these cell- and isoform-specific functions. In particular, the successful candidate will employ and adapt the Affinity-directed PROtein Missile system to target and manipulate specific proteins inside cells to enable the protein function to be controlled directly and acutely in a reversible manner in cells. This will be combined with elegant metabolic functional analyses developed by the Sakamoto group.

For informal enquiries please contact Gopal Sapkota (g.sapkota@dundee.ac.uk) and/or Kei Sakamoto (kei.sakamoto@sund.ku.dk).

For more details click here.


Post-doctoral position at the Institute of Genetics and Molecular and Cellular Biology

(Illkirch, France) in the team of Daniel Metzger

The project aims at understanding the molecular mechanisms underpinning the tissue specificity and the crosstalk between steroid hormone receptors through integrative analyses. Our team has recently developed projects based on genomewide characterization of transcription factor cistromes and transcriptomes in various tissues of genetically modified mice, thereby providing the unique opportunity to decipher the in vivo mode of action of nuclear receptors. The fellow’s tasks will be to identify tissuespecific receptor partners and characterize the associated epigenetic marks using cuttingedge technics like Atac-seq, Hi-C and Rapid Immunoprecipitation Mass Spectrometry (RIME). Genome-wide data obtained in various tissues will be strengthened in in vitro models to delineate these complex molecular mechanisms.

Candidates should send a curriculum vitae with a publication list, a short summary of research achievements and mastered techniques, as well as contact information of at least two references to metzger@igbmc.fr.

For more details click here.


Chercheur CDD biologie du muscle (sarcopénie et pathologie inflammatoire)

Offre pour un (.e) jeune cherch.eur (.euse) titulaire d’un doctorat pour un CDD de 12 à 15 mois.

Le poste est basé à l’université Clermont-Auvergne (Faculté de Médecine), dans l’unité de nutrition humaine, équipe alimentation santé musculaire et sarcopénie.

La personne sera impliquée dans un programme financé par la région Auvergne Rhône Alpes, visant à étudier les effets d’une approche pharmaco-nutritionnelle envers la fonte musculaire liée à l’induction d’une arthrite chez le rongeur. Les travaux concerneront l’étude du métabolisme énergétique, de l’inflammation, la physiologie musculaire. Le (la) cherch.eur (.euse) aura la charge d’effectuer des analyses d’expression de protéines, de gènes et de coupes histologiques et d’activité enzymatiques issus de prélèvements animaux, le traitement statistique des résultats. Des compétences dans les techniques de Western-Blot, une excellente rigueur scientifique afin de garantir la traçabilité des données sont indispensables. Des compétences en histologie et en PCR seront un atout important.

L’offre est à pouvoir dès que possible (envisager septembre, le temps de l’instruction du dossier).

Contacts responsables :
Frederic Capel
Frederic.capel@inrae.fr

Anne Tournadre
atournadre@chu-clermontferrand.fr


Scientist Position – Muscular disorders

Role & responsabilities:
• Design R&D programs and define adequate resources to complete the program successfully
• Propose new internal R&D programs
• Independently execute experiments and rigorously analyze and interpret the results
• Report results to CYTOO’s partners, write activity reports
• Drive at least one technician

If you are interested in this position, please send your resume and cover letter to eventre@cytoo.com.

Pour plus de détail cliquez ici.


Recrutement d’un.e Ingénieur.e en Protéomique

Poste à pourvoir à partir de septembre 2020

Nous souhaitons recruter, pour un contrat d’un an, un.e ingénieur.e spécialisé en protéomique avec une bonne connaissance de la biochimie des protéines, de la spectrométrie de masse, des systèmes chromatographiques et des principaux outils d’analyse de données de spectrométrie de masse. Des connaissances en bio-informatique seraient un plus. La personne recrutée prendra en charge nos prestations de recherche, en collaboration avec les chercheurs, ainsi que les projets de recherche et développements propres à la plateforme. Il ou elle devra faire preuve de rigueur, être autonome et avoir le sens de l’organisation. Les qualités relationnelles et le sens du service sont également essentielles au regard du caractère collaboratif inhérent au travail de plateforme technologique.

Missions
• Réaliser la préparation des échantillons protéiques, appliquer et adapter les techniques de biochimie
• Mettre en oeuvre les techniques de spectrométrie de masse couplée à la chromatographie liquide à haute performance
• Analyser les données LC-MS/MS
• Rédiger des rapports et des notes techniques
• Interagir avec les utilisateurs de la plateforme et les accompagner en amont et en aval de l’analyse protéomique
• Participer aux opérations de maintenance des équipements
• S’impliquer dans la démarche qualité

Merci d’envoyer un CV et une lettre de motivation avec des références à Solenne Chardonnet (solenne.chardonnet@sorbonne-universite.fr) et Gisèle Bonne (gisele.bonne@sorbonne-universite.fr)

Pour plus de détail cliquez ici.


PhD fellowship in Muscle Cell Biology at the Department of Biomedical Sciences

Faculty of Health and Medical Sciences
University of Copenhagen

Skeletal muscles are composed of numerous cells called muscle fibres, the basic units for generating muscle strength and, ultimately, movement. These fibres feature a large number of proteins, known as contractile proteins. In normal muscle fibres, these proteins are well-organised allowing an optimal production of force. In certain muscle disorders termed congenital myopathies, these proteins are not encoded properly and mutant molecules are then expressed. Patients with these molecular defects suffer from deleterious muscle weakness. The mechanisms by which these mutant proteins induce skeletal muscle weakness are currently not known. In the present research project, we, therefore, investigate how the mutants affect (i) protein function; (ii) the generation of the force of muscle fibres; and (iii) the consequences on the cellular architecture using unique biophysical and immuno-histochemical assays and tissue from human patients as well as animal models. Deciphering these mechanisms will allow us to design effective therapeutic interventions.

Start: October 1st 2020
Duration: 3 years as a PhD student

Your application must be submitted electronically and must include the following documents in PDF format:

1. Motivated letter of application (max. one page)
2. CV incl. education, experience, language skills and other skills relevant for the position
3. Master of Science diploma and transcript of records. If not completed, a certified/signed copy of a recent transcript of records or a written statement from the institution or supervisor will do
4. Publication list (if possible)

Application deadline: 19 July 2020, 23.59pm CET

Contact: julien.ochala@sund.ku.dk

For more details click here.


Postdoctoral Position in neurodegenerative diseases at Ecole Normale Supérieure

The Triller lab together with the Strick lab at the Institute of Biology at Ecole Normale Supérieure (IBENS) is seeking a postdoctoral scientist for a joint project focused on studying “New pathological mechanisms involved in Alzheimer’s disease: toward innovative therapeutic approaches.” Research in Antoine Triller’s lab is focused on membrane molecules which regulate the function of synapses by using cell and molecular biology together with single molecule imaging (sptPALM). Research in Terence Strick’s lab uses physical methods involving magnetic tweezers together with single-molecule imaging (NanoCOSM) to follow molecular interactions in real-time at the single molecule level.

Requirements:
• PhD or MD/PhD with a background in cell biology and neuroscience.
• Significant technical expertise in imaging and basic biophysics.
• Experience studying neurodegenerative disease is welcome but not required.
• Evidence of productivity from publications in peer-reviewed journals originating from Ph.D. or postdoctoral work.
• Excellent verbal and written communication skills in English
• Excellent organizational skills, self-motivation, and creativity.

Please send a cover letter explaining your relevant work experience and interests, a CV, and the contact information of three references to Dr Triller antoine.triller@ens.psl.eu
and Prof. Strick terence.strick@ens.psl.eu

For more details click here.


NEUROMUSCULAR DISEASE TRAINING PROGRAM

The Neuromuscular diseases Reference Centre of Nice University Hospital, an outstanding research center devoted to understanding neuromuscular diseases and development of innovative clinical therapies for neuromuscular disorders, is welcoming applications for position as neurologist fellow (FFI – Faisant Fonction D’Interne) in an international exchange program for 6 months to 1 year for training in neuromuscular diseases.

Duration: 6 months up to 1 year
Profile: Candidates should speak French and English to take part to clinical training and basic research projects.
This training is highly recommended for young neurologist in training wishing to spend a year in a foreign Institution and European PhD candidates

If interested, please send your CV at lahaut.p@chu-nice.fr.
For any question call at: 0033 (0) 4.92 03.89.11

For more details click here.


PhD position in DNA repair

Workplace: CEA 92265 Fontenay aux roses, Genome Instability and Nuclear Organisation Laboratory, IRCM, F. Jacob institute, UMR
Supervision : Dr Karine Dubrana
Type of contract: PhD student contract (CEA Funded)
Contract period: 36 months
Start date of the thesis: From October 1rst 2020

Objective of research: explore the mechanism insuring chromosome integrity at DNA double strand break sites

Application: The candidate must hold a an engineering or Master degree in biology. A background in molecular genetics and/or microscopy will be a plus.

Please send a single PDF with the following documents to Dr Karine Dubrana (karine.dubrana@cea.fr):
1. A detailed CV a,d motivation letter explaining why you want to join the lab, what you know and what you want to learn.
2. A short summary of your MSc research
3. At least two academic references (people who may be contacted)
4. Copies of university transcripts

For more details click here.


Full-time position – Clinical Pathologist

Laboratory of Clinical and Experimental Pathology, Nice University Hospital, Nice, France

The Laboratory of Clinical and Experimental Pathology (LPCE) invites applications for a full-time clinical pathologist position to replace a retiring Pathologist, starting date September 1 2019 if possible or later. Group currently consists of 6 Pathologists (2 full Professors, 2 Associated Professors, 1 Clinical Pathologist, and 1 Assistant Professor). ~4,000 Surgicals, Non-Gyn Cytology~1400, Molecular Path ~1000, Biobanking ~1000.

Minimum qualifications include an MD degree in anatomic pathology and a Research Background (e.g. Master in Life Sciences, publications). Additional interest or qualification in a subspecialty such as thoracic pathology (tumoral and non-tumoral), melanoma, head and neck, thyroid, lymphoma or cytology is very welcome.

The candidate needs to be willing to specialize or be specialized in neuromuscular pathology that will represent 30% of his workload (including training and participation to clinical staffs and meetings).

Please send CV to Professor Sabrina Sacconi in charge of evaluating candidate at: sacconi.s@chu-nice.fr

For more details click here.